On 23 October 1983 the first child was born by applying the in vitro fertilization procedure (IVF) at the University Hospital Center Zagreb, thus making Croatia the eighth country in the world where IVF was successfully applied. This was followed by the application of cryopreservation of embryos (1995), intracytoplasmic injection of spermatozoa (ICSI, 1995), aspiration of spermatozoa from epididymis and testis (PESA and TESA), as well as blastocyst transfer (1998), resulting in more than 4,500 children born by IVF and more than 12,000 children born by other procedures of assisted pregnancy all over Croatia.